Complement fixation activity of anti-U1 RNP and anti-Sm antibody by immunoblotting.
نویسندگان
چکیده
منابع مشابه
Anti-Sm and anti-RNP antibodies.
Among anti-nuclear antibodies, anti-Sm and anti-RNP antibodies are of the utmost importance in clinical practice. Anti-Sm antibodies are directed against 7 proteins (B/B', D1, D2, D3, E, F, G) that constitute the common core of U1, U2, U4 and U5 small nuclear ribonucleoprotein (snRNP) particles; B/B', D1 and D3 are more frequently targeted. Anti-RNP antibodies react with proteins (70 Kd, A, C) ...
متن کاملThe prevalence of antinuclear, anti-dsDNA, anti-Sm and anti-RNP antibodies in a group of healthy blood donors.
We studied the prevalence of antinuclear (ANA), anti-double stranded DNA (dsDNA), anti-Sm and anti-RNP antibodies in a group of 93 blood donors (age range: 18-58 years). Antinuclear and anti-ds DNA antibodies were detected by immunofluorescence (IF) using HEp2 cells and Crithidia luciliae as substrates, respectively, while anti-Sm and anti-RNP antibodies were assayed by ELISA. ANA was found in ...
متن کاملInterferon-α induction and detection of anti-ro, anti-la, anti-sm, and anti-rnp autoantibodies by autoantigen microarray analysis in juvenile dermatomyositis.
OBJECTIVE To evaluate serum interferon-α (IFNα) activity in the context of autoantibody profiles in patients with juvenile dermatomyositis (JDM). METHODS Sera from 36 patients with JDM were analyzed. Autoantibody profiles were determined by probing microarrays, which were fabricated with ∼80 distinct autoantigens, with serum and a Cy3-conjugated secondary antibody. Arrays were scanned and ana...
متن کاملComplement fixation by anti-dsDNA antibodies in SLE: measurement by radioimmunoassay and relationship with disease activity.
We have developed a sensitive, solid phase radioimmunoassay (RIA) to quantify the amount of complement (C') fixation by anti-double-stranded DNA (anti-dsDNA) antibodies and have studied sera from 48 patients with systemic lupus erythematosus (SLE). 46% of the patients were positive in this assay. There was no correlation with serum C' levels, and only weak correlation with anti-dsDNA activity a...
متن کاملAnti-nociceptive and anti-inflamatory activity of olive fruit extract
In Iranian traditional medicine, olive has been reported to have analgesic and anti-inflamatory effects. In the present study the anti-nociceptive and anti-inflamatory effects of olive fruit has been studied. In this study the methanolic and aqueous extract of the fruit with HER (Herbal to Extract Ratio) of 4.96% and 4.84% has been used and analgesic effect of this extract has been compared wit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Japanese Journal of Clinical Immunology
سال: 1991
ISSN: 0911-4300,1349-7413
DOI: 10.2177/jsci.14.395